2ZOM
Crystal structure of CutA1 from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-04 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 127.214, 127.214, 121.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.610 - 3.020 |
| R-factor | 0.18237 |
| Rwork | 0.178 |
| R-free | 0.26674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zfh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.204 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.628 | 3.100 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.061 | 0.231 |
| Number of reflections | 10138 | |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.3 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 1.8M Ammonium sulfate, 0.09M tri-Sodium citrate dihydrate pH5.6, 0.18M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
