2ZOF
Crystal structure of mouse carnosinase CN2 complexed with MN and bestatin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.8926, 1.8941, 1.7926 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.496, 199.181, 55.215 |
Unit cell angles | 90.00, 118.92, 90.00 |
Refinement procedure
Resolution | 43.030 - 2.300 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.424 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.058 | 0.235 |
Number of reflections | 39799 | |
Completeness [%] | 86.8 | 30.9 |
Redundancy | 6.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 20% PEG 3350, 20% GLYCEROL, 0.2M HF, 25MH TRIS-HCL, 50MM NACL, 0.2MM MNCL2, 1MM DTT, 30MM BESTATIN, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 277K |