2ZOA
Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) COLLECTED AT 1.280 ANGSTROM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2804 |
Spacegroup name | P 21 3 |
Unit cell lengths | 164.343, 164.343, 164.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.500 - 2.400 |
R-factor | 0.15953 |
Rwork | 0.159 |
R-free | 0.17893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dxn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.607 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 57620 | |
<I/σ(I)> | 23.1 | 2.4 |
Completeness [%] | 99.6 | 98.5 |
Redundancy | 5.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |