2ZO7
Crystal Structure of a Kusabira-Cyan Mutant (KCY-R1), a Cyan/Green-Emitting GFP-Like Protein
Replaces: 2EJIExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.689, 45.016, 51.158 |
Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
Resolution | 18.700 - 1.580 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zo6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.060 | 0.257 |
Number of reflections | 29798 | |
<I/σ(I)> | 5 | |
Completeness [%] | 98.0 | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 35-40% PEG 8000, 0.2M (NH4)2SO4, 0.1M SODIUM CACODYLATE, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 291K |