Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.754, 42.804, 50.699 |
| Unit cell angles | 90.00, 96.28, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.400 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mou |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.042 | 0.256 |
| Number of reflections | 36604 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 91.6 | 62.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 20% PEG 3350, 0.2M LICL, 20MM TRIS/HCL, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
