2ZO4
Crystal structure of metallo-beta-lactamase family protein TTHA1429 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.2822, 1.2829, 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.286, 62.885, 87.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25696 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 19700 | |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100mM Bicine-NaOH, 25% (w/v) PEG 4000, 5% (v/v) 2-propanol, 5mM zinc acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
