2ZNH
Crystal Structure of a Domain-Swapped Serpin Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.350, 99.880, 92.460 |
| Unit cell angles | 90.00, 100.62, 90.00 |
Refinement procedure
| Resolution | 43.770 - 2.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e04 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.148 | 0.502 |
| Number of reflections | 31431 | |
| <I/σ(I)> | 4.4 | 3.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 20% PEG3350, 0.2M tri-potassium citrate, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
