2ZL6
Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.512, 83.512, 162.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.450 - 1.430 |
| R-factor | 0.18023 |
| Rwork | 0.179 |
| R-free | 0.20048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ihm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.730 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.070 | 0.532 |
| Number of reflections | 121303 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 5.38 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 293 | 0.1M Citric Acid(pH 3.8), 0.2M Ammonium Nitrate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
