2ZL3

Crystal structure of H.pylori ClpP S99A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-20
Detector	BRUKER PROTEUM 300
Wavelength(s)	1.28362
Spacegroup name	P 21 21 21
Unit cell lengths	91.739, 166.411, 186.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.810
R-factor	0.212
Rwork	0.212
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zl0
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	69011

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	32% PEG 400, 0.1M HEPES, 0.35M magnesium chloride, pH 7.5, Microbatch, temperature 298K