2ZL1

MP1-p14 Scaffolding complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 21 21 21
Unit cell lengths	44.290, 62.440, 74.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.000
R-factor	0.20515
Rwork	0.203
R-free	0.25602
RMSD bond length	0.013
RMSD bond angle	1.402
Data scaling software	d*TREK (9.5L)
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	32.540	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.056	0.032	0.342
Total number of observations		8153	8040
Number of reflections	14618
<I/σ(I)>	12.7	30.1	3.9
Completeness [%]	100.0	100	100
Redundancy	5.52	5.12	5.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	11%(w/v) PEG 3350, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 295K