2ZKW
Crystal structure of human Cu-Zn superoxide dismutase mutant G85R in space group P21
Replaces: 2YQVExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.616, 46.233, 52.818 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 33.460 - 1.900 |
Rwork | 0.193 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hl5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 17839 | |
<I/σ(I)> | 10.367 | 4.25 |
Completeness [%] | 97.0 | 92.4 |
Redundancy | 3.583 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.2M Ammonium iodide, 20%(w/v) PEG3350, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |