2ZKO
Structural basis for dsRNA recognition by NS1 protein of human influenza virus A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.707, 57.218, 83.709 |
| Unit cell angles | 90.00, 105.95, 90.00 |
Refinement procedure
| Resolution | 40.860 - 1.700 |
| R-factor | 0.19075 |
| Rwork | 0.189 |
| R-free | 0.22965 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ail |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 28724 | |
| <I/σ(I)> | 30.7 | 2.3 |
| Completeness [%] | 99.5 | 95.5 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Magnesium acetate, MES, Ammonium sulfate, ATP, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Magnesium acetate | ||
| 10 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | Ammonium sulfate | ||
| 4 | 1 | 1 | ATP | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | Magnesium acetate | ||
| 7 | 1 | 2 | MES | ||
| 8 | 1 | 2 | Ammonium sulfate | ||
| 9 | 1 | 2 | ATP |
