2ZKI

Crystal structure of hypothetical Trp repressor binding protein from Sul folobus tokodaii (ST0872)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-03
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	105.624, 194.995, 100.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.850 - 2.900
R-factor	0.20507
Rwork	0.202
R-free	0.25695
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zwk
RMSD bond length	0.006
RMSD bond angle	0.846
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.083	0.240
Number of reflections	43496
<I/σ(I)>	18.9
Completeness [%]	93.6	93.2
Redundancy	6.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8M ammonium sulfate, 5% iso-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K