2ZKI
Crystal structure of hypothetical Trp repressor binding protein from Sul folobus tokodaii (ST0872)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-03 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.624, 194.995, 100.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.850 - 2.900 |
| R-factor | 0.20507 |
| Rwork | 0.202 |
| R-free | 0.25695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zwk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.083 | 0.240 |
| Number of reflections | 43496 | |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 93.6 | 93.2 |
| Redundancy | 6.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8M ammonium sulfate, 5% iso-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
