2ZJD
Crystal Structure of LC3-p62 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.559, 39.592, 78.011 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.560 |
| R-factor | 0.20281 |
| Rwork | 0.201 |
| R-free | 0.23766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ugm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.298 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.850 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.054 | 0.310 |
| Number of reflections | 39943 | |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 98.4 | 86 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 0.1M HEPES pH 7.5, 23% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
