2ZJB

Crystal structure of the human Dmc1-M200V polymorphic variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	MAR CCD 225 mm
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	124.089, 124.089, 217.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.500
Rwork	0.294
R-free	0.35100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v5w
RMSD bond length	0.008
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.500	3.500
Number of reflections	11293
<I/σ(I)>	33.1	6
Completeness [%]	100.0	100
Redundancy	13.1	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	0.1M sodium citrate, 50mM MgCl2, 8% PEG 2000 MME, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K