2ZIX
Crystal structure of the Mus81-Eme1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.814, 85.814, 176.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.28815 |
| Rwork | 0.285 |
| R-free | 0.34554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ziu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.131 | 0.303 |
| Number of reflections | 10913 | |
| <I/σ(I)> | 17.1 | 3.6 |
| Completeness [%] | 97.4 | 93.2 |
| Redundancy | 9.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M BTP, 1.25M Li2SO4, 100mM MgCl2, 2mM CdCl2, VAPOR DIFFUSION, HANGING DROP, pH 6.5, temperature 277K |
