2ZIV
Crystal structure of the Mus81-Eme1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.439, 88.439, 169.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 2.700 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.274 |
| Number of reflections | 26740 | |
| <I/σ(I)> | 36.6 | 3.8 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 6.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1M Na-Acetate, 2.4M ammonium chloride, VAPOR DIFFUSION, HANGING DROP, pH 5.0, temperature 277K |
