2ZIT
Structure of the eEF2-ExoA-NAD+ complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 326.920, 69.241, 190.483 |
Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
Resolution | 19.899 - 3.000 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26600 |
Starting model (for MR) | eEF2-ExoA(E546H)-NAD+ |
RMSD bond length | 0.002 |
RMSD bond angle | 0.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.164 | 0.583 |
Number of reflections | 84042 | |
<I/σ(I)> | 4.6 | 1.4 |
Completeness [%] | 98.4 | 86.9 |
Redundancy | 3.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7% PEG-10000, 3.5mM MPD, 100mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |