2ZIL

Crystal Structure of Human Lysozyme from Urine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-02-23
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.700, 61.000, 34.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.520 - 1.800
R-factor	0.17305
Rwork	0.170
R-free	0.22671
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i1z
RMSD bond length	0.013
RMSD bond angle	1.411
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.520
High resolution limit [Å]	1.800
Rmerge	0.084
Number of reflections	11190
Completeness [%]	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	2.5M sodium chloride, 0.03M phosphate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K