2ZIJ

Crystal Structure of Human Lysozyme Expressed in E. coli.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1999-04-07
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.600, 61.000, 33.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.500 - 1.900
R-factor	0.17082
Rwork	0.167
R-free	0.23541
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i1z
RMSD bond length	0.014
RMSD bond angle	1.522
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.500
High resolution limit [Å]	1.900
Rmerge	0.078
Number of reflections	8599
Completeness [%]	88.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	2.5M sodium chloride, 0.03M phosphate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K