2ZIH

Crystal Structure of Yeast Vps74

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926, 0.97942, 0.94927
Spacegroup name	P 31 2 1
Unit cell lengths	102.688, 102.688, 292.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.990 - 2.800
R-factor	0.237
Rwork	0.233
R-free	0.30200
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.640
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.093	0.050	0.342
Number of reflections	43558
<I/σ(I)>	11.3
Completeness [%]	97.4	97.4	83.8
Redundancy	5.2	5.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	8% PEG 3350, 5% ethylene glycol, 50mM MES, 50mM NaCl, 50mM CaCl2, 10mM EGTA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	8% PEG 3350, 5% ethylene glycol, 50mM MES, 50mM NaCl, 50mM CaCl2, 10mM EGTA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K