2ZIE
Crystal Structure of TTHA0409, Putatative DNA Modification Methylase from Thermus thermophilus HB8- Selenomethionine derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97887, 0.90000, 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.577, 58.252, 80.908 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.500 |
| Rwork | 0.222 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.291 |
| Number of reflections | 19995 | |
| <I/σ(I)> | 27.2 | 5.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 0.1M Tris HCl, 38% Ethanol, 25mM Mg Chloride, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
