2ZI0
Crystal structure of Tav2b/siRNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.455, 122.043, 28.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.850 - 2.820 |
| R-factor | 0.21648 |
| Rwork | 0.214 |
| R-free | 0.27599 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.527 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 7336 | |
| <I/σ(I)> | 20.8 | 4.44 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25% PEG 1500, 1.0M Ammonium formate, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | Ammonium formate | ||
| 3 | 1 | 1 | PEG | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | Ammonium formate | ||
| 7 | 1 | 2 | PEG | ||
| 8 | 1 | 2 | HOH |
