2ZHP

Crystal structure of bleomycin-binding protein from Streptoalloteichus hindustanus complexed with bleomycin derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	39.992, 53.868, 125.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.110 - 1.600
Rwork	0.187
R-free	0.21600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.075	0.313
Number of reflections	36738
<I/σ(I)>	47.2	8.3
Completeness [%]	100.0	100
Redundancy	13.7	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	PEG550mme, zinc sulfate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	PEG550mme, zinc sulfate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K