2ZHM

Crystal structure of human galectin-9 N-terminal CRD in complex with N-acetyllactosamine trimer (crystal 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-17
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	35.217, 67.964, 219.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.900 - 1.840
R-factor	0.185
Rwork	0.182
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.900	1.920
High resolution limit [Å]	1.840	1.850
Number of reflections	45532
Completeness [%]	98.9	99.5
Redundancy	10.9	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	200MM AMMONIUM FLUORIDE, 20% PEG 3350, pH 6.20, VAPOR DIFFUSION, SITTING DROP, temperature 293K