2ZH2
Complex structure of AFCCA with tRNAminiDAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.983, 57.983, 440.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.940 - 2.660 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2drb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.760 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Number of reflections | 23187 | |
| <I/σ(I)> | 50.5 | 11.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 50mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM NH4SO4, pH 7.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 10 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | PEG 3550 | ||
| 3 | 1 | 1 | Tri-lithium citrate | ||
| 4 | 1 | 1 | NH4SO4 | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | PEG 3550 | ||
| 8 | 1 | 2 | Tri-lithium citrate | ||
| 9 | 1 | 2 | NH4SO4 |
