2ZGM
Crystal structure of recombinant Agrocybe aegerita lectin,rAAL, complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.090, 58.820, 57.910 |
Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
Resolution | 29.460 - 1.900 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zgl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.670 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.119 |
Number of reflections | 20381 | |
<I/σ(I)> | 22.6 | 9.2 |
Completeness [%] | 97.6 | 83.8 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M Ammonium Sulfate, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |