2ZGK
Crystal structure of wildtype AAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 124.040, 124.040, 56.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.850 - 3.000 |
| R-factor | 0.282 |
| Rwork | 0.253 |
| R-free | 0.28300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.510 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.046 | 0.216 |
| Number of reflections | 5762 | |
| <I/σ(I)> | 22.4 | 11.7 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 5% glycerol, 1.7M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
