2ZGI
Crystal Structure of Putative 4-amino-4-deoxychorismate lyase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-02-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97892, 0.979, 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.613, 133.585, 141.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.19618 |
| Rwork | 0.193 |
| R-free | 0.24927 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0061) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.063 | 0.578 |
| Number of reflections | 91735 | |
| Completeness [%] | 96.8 | 72 |
| Redundancy | 13.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.3M Li2SO4, 0.1M Na Hepes, pH 8.0, 25% Gly (cryo), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 2 |
