2ZGD
Asn-hydroxylation stabilises the ankyrin repeat domain fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 48.887, 48.887, 67.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.290 - 1.900 |
Rwork | 0.173 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n0q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.134 | 0.112 | 0.392 |
Number of reflections | 7197 | ||
<I/σ(I)> | 7.4 | 3.75 | |
Completeness [%] | 93.3 | 86.2 | 95.8 |
Redundancy | 4.9 | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG 400, 100mM Na Acetate pH 4.6, 100mM CdCl2, vapor diffusion, sitting drop, temperature 293K |