2ZFU
Structure of the methyltransferase-like domain of nucleomethylin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2006-04-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 |
Unit cell lengths | 43.810, 43.810, 403.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.520 - 2.000 |
R-factor | 0.19361 |
Rwork | 0.192 |
R-free | 0.23066 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.237 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.270 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.072 | 0.284 |
Number of reflections | 28996 | |
Completeness [%] | 98.0 | 92 |
Redundancy | 11 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |