2ZFE
Crystal structure of bacteriorhodopsin-xenon complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.410, 102.410, 112.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 12458 | |
| <I/σ(I)> | 27.8 | 5.5 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 14.6 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 283 | 2.2M ammonium sulfate, 0.1M sodium citrate, 0.16M potasium chloride, pH5.2, membrane fusion method, temperature 283K |
