2ZFC

X-ray crystal structure of an engineered N-terminal HIV-1 GP41 trimer with enhanced stability and potency

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	103.569, 103.569, 44.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.221
R-free	0.26600
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	28563
<I/σ(I)>	44	1.6
Completeness [%]	95.5	68.6
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	10%(v/v) iso-propanol, 0.1M Na Citrate pH 5.6, 10%(w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K