2ZFB
Crystal structure of parrot hemoglobin (Psittacula krameri) at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.676, 64.268, 56.396 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 14.390 - 3.000 |
| R-factor | 0.19822 |
| Rwork | 0.190 |
| R-free | 0.27448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c40 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.689 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.950 | 14.950 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 6543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 10% PEG 3350, phosphate buffer pH 7.5, VAPOR DIFFUSION, temperature 291K |
