2ZFA
Structure of Lactate Oxidase at pH4.5 from AEROCOCCUS VIRIDANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 132.595, 132.595, 90.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.090 - 1.810 |
| R-factor | 0.18557 |
| Rwork | 0.184 |
| R-free | 0.20757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.070 | 0.359 |
| Number of reflections | 71313 | |
| <I/σ(I)> | 61.3 | 6.9 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 14.8 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 40% Ethylene Glycol, 100mM acetate buffer (pH 4.5), VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
