2ZF9
Crystal structure of a type III cohesin module from the cellulosomal ScaE cell-surface anchoring scaffoldin of Ruminococcus flavefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.560, 69.250, 93.030 |
Unit cell angles | 90.00, 123.37, 90.00 |
Refinement procedure
Resolution | 23.690 - 1.950 |
R-factor | 0.16413 |
Rwork | 0.162 |
R-free | 0.21122 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.421 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.800 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.078 | 0.372 |
Number of reflections | 60499 | |
<I/σ(I)> | 29.9 | 2.26 |
Completeness [%] | 98.0 | 89.8 |
Redundancy | 6.73 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Bis-Tris, 23% PEG 3350, 0.1M calcium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |