2ZEZ
Family 16 Carbohydrate Binding Module-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 99.425, 99.525, 139.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.23459 |
Rwork | 0.233 |
R-free | 0.27203 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zew |
RMSD bond length | 0.011 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 51462 | |
<I/σ(I)> | 35.3 | 11.6 |
Completeness [%] | 93.5 | 85.8 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 3.5M sodium formate pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |