2ZEY
Family 16 carbohydrate binding module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-18 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.094, 74.094, 116.086 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.17177 |
| Rwork | 0.170 |
| R-free | 0.21231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zew |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 19044 | |
| <I/σ(I)> | 41.3 | 8.2 |
| Completeness [%] | 98.9 | 91.7 |
| Redundancy | 5.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.5M ammonium sulfate, 100mM MEX pH6.5, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
