2ZEX
Family 16 carbohydrate binding module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-22 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 32.466, 48.829, 49.356 |
Unit cell angles | 62.03, 84.88, 85.95 |
Refinement procedure
Resolution | 20.000 - 1.200 |
R-factor | 0.15679 |
Rwork | 0.156 |
R-free | 0.17864 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zew |
RMSD bond length | 0.008 |
RMSD bond angle | 1.241 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.180 | 1.180 |
Number of reflections | 82565 | |
<I/σ(I)> | 33.3 | 6 |
Completeness [%] | 94.2 | 87.5 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 30% PEG3350, 100mM Tris-HCL pH8.3, 200mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |