2ZDO
Crystal structure of IsdG-N7A in complex with hemin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 46.585, 58.631, 59.692 |
Unit cell angles | 77.57, 74.39, 75.15 |
Refinement procedure
Resolution | 56.800 - 1.800 |
R-factor | 0.19997 |
Rwork | 0.198 |
R-free | 0.23446 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sqe |
RMSD bond length | 0.012 |
RMSD bond angle | 1.389 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.800 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 49184 | |
<I/σ(I)> | 6.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |