2ZCY
yeast 20S proteasome:syringolin A-complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.051, 301.964, 143.835 |
| Unit cell angles | 90.00, 112.87, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.940 |
| High resolution limit [Å] | 2.800 | 2.890 |
| Rmerge | 0.135 | 0.525 |
| Number of reflections | 252555 | |
| <I/σ(I)> | 9.1 | 2.2 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 294 | 0.1M MES, 12% MPD, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
