2ZCX
Crystal structure of TetR family transcriptional regulator SCO7815
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-13 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 2.29 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.068, 72.068, 90.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.220 |
Rwork | 0.222 |
R-free | 0.24750 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 12269 | |
<I/σ(I)> | 45.705 | 8.064 |
Completeness [%] | 99.6 | 100 |
Redundancy | 27 | 22.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.8M potassium sodium tartrate, 0.1M Tris-HCl, 1% PEG5000MME, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |