2ZCW
Crystal Structure of TTHA1359, a Transcriptional Regulator, CRP/FNR family from Thermus thermophilus HB8
Replaces: 2COHExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-07 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9, 0.97874, 0.97939 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.050, 54.050, 147.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.400 - 1.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.046 | 0.266 |
| Number of reflections | 39799 | |
| <I/σ(I)> | 50.7 | 7.7 |
| Completeness [%] | 99.6 | 96.1 |
| Redundancy | 13.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 31% MPD, 0.1M sodium acetate, 20mM calcium chloride, pH5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
