2ZCV

Crystal structure of NADPH-dependent quinone oxidoreductase QOR2 complexed with NADPH from escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-10
Detector	BRUKER PROTEUM 300
Spacegroup name	P 32 2 1
Unit cell lengths	81.284, 81.284, 77.506
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.520 - 2.300
R-factor	0.217
Rwork	0.217
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zcu
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	SMART
Data scaling software	SAINT (& PROSCALE)
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	13535
Completeness [%]	86.9	82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	285	PEG 4000, COPPER CHLRORIDE, HEPES, AMMONIUM SULFATE, pH 7.50, EVAPORATION, temperature 285K