2ZCL
Crystal structure of human prostate specific antigen complexed with an activating antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-09-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.978, 87.978, 238.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.480 - 3.250 |
| R-factor | 0.20379 |
| Rwork | 0.201 |
| R-free | 0.25098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zch |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.742 |
| Data reduction software | MOSFLM |
| Data scaling software | PROCESS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.040 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.144 | 0.467 |
| Number of reflections | 15532 | |
| <I/σ(I)> | 14.9 | 4.9 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | crystals grown in 14% MPEG550, 100mM HEPES (pH7.2), then soaked in 33% PEG400, 100mM MES (pH5.5), 10mM ZnCl2, 0.28mg/ml Mu-KGISSQY-AFC, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
