2ZCG
Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.929, 104.087, 62.420 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 15.010 - 2.223 |
| R-factor | 0.20478 |
| Rwork | 0.201 |
| R-free | 0.27095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f84 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.010 | 2.280 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.102 | 0.319 |
| Number of reflections | 32849 | |
| <I/σ(I)> | 9.6 | 3.2 |
| Completeness [%] | 99.0 | 95.4 |
| Redundancy | 4.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Protein at 10mg/ml (in 25mM HEPES and 150mM NaCl)combined with equal volume of solution containing 100mM sodium acetate and 2M sodium formate., pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
