2ZC0
Crystal structure of an archaeal alanine:glyoxylate aminotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-01 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.694, 112.519, 207.287 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.400 - 2.300 |
Rwork | 0.236 |
R-free | 0.26100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 79851 | |
<I/σ(I)> | 12.4 | 5.2 |
Completeness [%] | 98.0 | 87.8 |
Redundancy | 7.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |