2ZBV
Crystal structure of uncharacterized conserved protein from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9790 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.200, 136.697, 186.312 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.050 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2YYP |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.091 | 0.280 |
Number of reflections | 58169 | |
<I/σ(I)> | 34.1 | 3.5 |
Completeness [%] | 98.4 | 86.3 |
Redundancy | 12.6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 0.1M Sodium cacodylate pH 6.3, 31% 2-ethoxyethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |