2ZBU
Crystal structure of uncharacterized conserved protein from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9000, 0.9794 |
| Spacegroup name | H 3 |
| Unit cell lengths | 120.476, 120.476, 227.115 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.344 |
| Number of reflections | 71732 | |
| <I/σ(I)> | 25.8 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Sodium cacodylate pH6.5, 35% 2-ethoxyethanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
