2ZBR
Crystal structure of ribosomal protein L11 methyltransferase from Thermus thermophilus in complex with S-adenosyl-ornithine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.279, 75.480, 62.042 |
| Unit cell angles | 90.00, 130.67, 90.00 |
Refinement procedure
| Resolution | 31.200 - 1.900 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24700 |
| Structure solution method | RIGID-BODY REFINEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22589 | |
| <I/σ(I)> | 35.5 | 4.3 |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | MES, ammonium sulfate, dioxane, 1,6-hexanediol, pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
